紫外-可见吸收、荧光光谱研究壳核型CdTe/CdS量子点与盐酸药根碱的相互作用及其应用

Determination of Jatrorrhizine Hydrochloride by Core-shell CdTe/CdS Quantum Dots with Fluorescence Quenching Method

合成了巯基乙酸(TGA)修饰的壳核型CdTe/CdS量子点(TGA-CdTe/CdS QDs)。利用紫外-可见光谱吸收、荧光光谱研究TGA-CdTe/CdS QDs与盐酸药根碱(JH)的相互作用机理。在pH值为7.4的tris-HCl缓冲溶液介质中,QDs与JH相互作用后使QDs的荧光呈线性猝灭,并有良好的线性关系(r=0.999 1),线性范围0.011～10 mg/L,检出限(3σ)为3.3×10-3mg/L,因此可以作为一种快速、简便、定量测定盐酸药根碱的新方法。

The mercaptoacetic acid （TGA） stabilized core-shell CdTe/CdS quantum dots （TGA-CdTe/CdS QDs） were synthesized using as stabilizer. A new method for the determination of jatrorrhizine hydrochloride has been developed based on the quenching of the fluorescence of TGA-CdTe/CdS QDs at excitation wavelength of 340 nm. The paper utilized fluorescence spectrometry and ultraviolet-visible （UV-Vis） absorption spectra to study the interaction between TGA-CdTe/CdS QDs and jatrorrhizine hydrochloride （JH）. Under optimized conditions, in which at the pH value is 7.4 using tris-HC1 buffer solution, the quenched fluorescence intensity of TGA-CdTe/CdS QDs was linearly proportional with a correlation coefficient r = 0. 9991, and the limits of detection were in the range of 0. 011- 10 mg/L and the detection limit for JH（3σ） was 3.3×10-3mg/L. JH could be conveniently and quantitatively detemined using this new method.