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电化学研究聚乙二醇单甲醚接枝壳聚糖的pH敏感性及对细胞色素c的控制释放 被引量：2

pH Sensitivity of Monomethoxypoly(ethylene glycol)grafted-chitosan and Its Controlled Delivery of Cytochrome c via Electrochemical Method
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摘要 采用电化学沉积法在Au电极上制备了聚乙二醇单甲醚接枝壳聚糖(mPEG-g-CS)电沉积膜及其与细胞色素c(Cyt c)复合膜。循环伏安(CV)和交流阻抗(EIS)等电化学手段研究表明,mPEG-g-CS膜具有良好的pH敏感性,通过调节溶液的pH值,可以改变电化学探针[Fe(CN)6]3-/4-在电极表面的电子转移速率;Cyt c在mPEG-g-CS膜内可以实现直接电子转移,该复合膜电极对H2O2的响应快速而灵敏,说明mPEG-g-CS能较好的保持Cyt c的生物活性。通过控制溶液的pH值,实现了Cyt c在mPEG-g-CS膜内的可逆释放和关闭。 A pH-sensitive monomethoxypoly （ethylene glycol）-grafted-chitosan （mPEG-g-CS） film was used as a matrix for reversible immobilization of cytochrome c （Cyt c ） on a Au surface through a novel electrodeposition process. The pH-sensitive behavior of the mPEG-g-CS film was demonstrated by cyclic voltammetry （ CV ） and electrochemical impedance spectroscopy （ EIS ） measurements. Electrochemical co-deposited Cyt c and mPEG-g-CS exhibited the direct electrochemical behavior which implied that mPEG-g- CS could well control the delivery of Cyt c under different pH.
作者 刘倩 钱静 王坤 李夜平 吴向阳
机构地区 江苏大学化学化工学院 江苏大学药学院
出处 《应用化学》 CAS CSCD 北大核心 2012年第3期340-345,共6页 Chinese Journal of Applied Chemistry
基金 国家自然科学基金(20875039和21075054) 现代农业装备与技术省部共建教育部重点实验室开放基金(NZ200809) 江苏大学高级人才基金(06JDG016)资助项目
关键词 聚乙二醇单甲醚接枝壳聚糖 PH敏感性 细胞色素C 直接电化学 控制释放 monomethoxypoly （ethylene glycol ）-grafted-chitosan, pH sensitivity, cytochrome c, direct electrochemistry, controlled delivery
分类号 O657.1 [理学—分析化学]
  • 相关文献

参考文献15

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共引文献5

同被引文献31

  • 1杨心督,陈汉,张惠珠,高福平,王银松,张其清.聚乙二醇单甲醚接枝壳聚糖自组装纳米球的制备[J].中国新药杂志,2007,16(21):1780-1783. 被引量：3
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应用化学
2012年 第3期

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