黄花草木犀总皂苷提取纯化工艺优选

Optimization of Eextraction Process and Purification Process of Total Saponins from Melilotus officinalis

目的:优选黄花草木犀总皂苷最佳提取纯化工艺。方法:以黄花草木犀总皂苷含量为考察指标,采用正交试验,研究黄花草木犀最佳提取和纯化工艺。结果:最佳提取工艺为加14倍量70%乙醇提取3次,每次1 h;最佳纯化工艺为浓缩浸膏上D101型大孔树脂柱,洗脱剂70%乙醇,吸附流速1 mL.min-1,洗脱流速3 mL.min-1,洗脱体积6 BV。结论:黄花草木犀总皂苷优选的提取纯化工艺简便、稳定、可靠。

Objective：To optimize optimum extraction process and purification process of total saponins from Melilotus officinalis.Method：Orthogonal test was designed for investigating optimum extraction and purification technology for M.officinalis with the content of total saponins as index,respectively.Result：Optimum extraction process of total saponins were：extracted 3 times with 14 times the amount of 70% ethanol for 1 h each time,extraction solvent to reflux for 1.0 hour and 3 times.Optimum purification process was：concentrated extract passed through D101 resin with eluting by 6.0 BV 70% ethanol,elution flow rate was 3.0 mL·min-1,adsorption flow rate was 1.0 mL·min-1.Conclusion：This optimized process was simple,stable and reliable.