摘要
目的:采用高效液相色谱法测定散结镇痛胶囊中黄酮类成分的指纹图谱。方法:采用50%甲醇超声提取黄酮类成分进行分析检测。色谱条件为Waters Symmetry@C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-水,梯度洗脱;检测波长275 nm;流速1.0 mL.min-1;柱温30℃。采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》(2004年A版)软件,对10批不同批次的散结镇痛胶囊中黄酮类成分指纹图谱进行相似度计算。结果:指纹图谱中标记了15个共有峰,共有峰面积之和大于总峰面积的95%,利用指纹图谱相似度测试软件,测定10批散结镇痛胶囊中黄酮类成分指纹图谱相似度均在0.99以上。结论:方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于散结镇痛胶囊质量的综合评价。
Objective：To establish the chromatographic fingerprint analysis for the quality control of the content of flavonoids in Sanjie Zhentong capsules.Method： Under the operation of ultrasonic extraction,the flavonoids from Sanjie Zhentong capsules were extracted in the 50% methanol.With Waters Symmetry@ C18（4.6 mm×250 mm,5 μm）column,and gradient elution was performed by mobile phase containing acetonitrile and water.The flow rate was 1.0 mL·min-1 and the detection wave length was at 203 nm.The temperature of column was maintained at 30 ℃.And data were analyzed by ＇Similarity evaluation software for chromatographic Fingerprint of traditional Chinese medicine＇ software to compare the quanlity of 11 batches of capsules samples.Result： Fifty common peaks were selected in chromatography,and all the common peaks were separated effectively.Conclusion： The accuracy,repeatability,and stability of this method were satisfied.The method developed can be used to evaluate the quality of Sanjie Zhentong capsules synthetically.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期58-61,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部重大新药创制项目(2011ZX09201-201-20)
