摘要
目的:建立HPLC测定左归丸中齐墩果酸、熊果酸含量的方法。方法:采用反相高效液相色潽法,Sunfire C18柱,甲醇-水(85∶15)(磷酸二氢钠调pH 6.5),流速1.0 mL.min-1,检测波长215 nm,柱温25℃。结果:齐墩果酸在0.05～1.0μg线性关系良好(r=0.999 9),回收率97.7%(RSD 1.39%,n=6);熊果酸在0.1～2.0μg线性关系良好(r=0.999 9),回收率98.3%(RSD 0.82,n=6)。结论:方法可用于左归丸中熊果酸、齐墩果酸的含量测定。
Objective：To establish a HPLC method for the determination of ursolic acid and oleanolic acid in Zuogui Wan.Method： The method was applied with Sunfire C18 column by methanol-water（25∶75,adjust pH to 6.5 with sodium dihydrogen phosphate） as the mobile phase,with the flow rate 1.0 mL·min-1.The column temperature was maintained at 25 ℃ and the UV detection wavelength was set at 215 nm.Result： Oleanolic acid was linear in the range of 0.05-1.0 μg（r=0.999 9）,the average recovery was 97.7%（RSD 1.39,n=6）.Ursolic acid was linear in the range of 0.1-2.0 μg（r=0.999 9）,the average recovery was 98.3%（RSD 0.82,n=6）.Conclusion： The method can be used for determination of oleanolic acid and ursolic acid in Zuogui Wan.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期92-94,共3页
Chinese Journal of Experimental Traditional Medical Formulae
