双波长光度法测定苦参、黄芪中的铜含量

Determining Copper in Sophora Flavescens Radix and Astrangali Radix by Double-wavelength Spectrophotometriy

目的:建立了双指示剂催化动力学光度法测定痕量铜的新方法。方法:在酸性介质中,铜离子催化甲基橙和亚甲基蓝双指示剂的氧化褪色反应,通过催化体系和非催化体系吸光度变化与铜离子浓度之间的关系,建立了测定痕量铜的方法。结果:铜(Ⅱ)在0.005 0～0.160 mg.L-1,与催化反应速度成线性关系可以进行含量的测定。结论:该方法方便、准确、重复性好,可以作为测定了黄芪苦参中微量的铜(Ⅱ)元素的方法。

Objective：To determine copper by the double-wavelength spectrophotometric.Method：A new double-wavelength spectrophotometric method has been developed for the determination of copper.Result：The experimental results shown in the best condition the apparent molar absorptivity of copper was 0.005 0-0.160 mg·L-1.Conclusion：The method is applied to the direct determination of copper in Chinese traditional medicine of with satisfactory results.