基于显微图像的血虚证大鼠肠系膜微循环变化研究

Study on Changes of Mesenteric Microcirculation in Rats with Syndrome of Deficiency of Blood Based on Micro Image Analysis System

目的:观察血虚证大鼠肠系膜微循环变化,并测量其血液流速。方法:通过皮下注射乙酰苯肼(APH)制备血虚证大鼠模型,运用BI-2000医学图像分析系统观测血虚大鼠肠系膜微循环损伤的变化,采集微循环显微图像,并利用LabVIEWIMAQ VISION平台进行图像处理分析,提供一种跟踪白细胞团的微循环血液流速测量方法。结果:①血虚大鼠肠系膜微循环血色暗淡,微血管变形,管壁受损;②测得的大鼠肠系膜微循环血液流速与现代文献研究十分吻合,精确度提高,再现性良好。③血虚模型组大鼠肠系膜微循环血液流态呈粒线流甚至聚集流改变,且血液流速较正常组明显减慢,具有显著性差异(P<0.05)。结论:血虚大鼠微循环存在供血不足、血流瘀滞、管壁损伤和渗出出血等多重病理改变;运用LabVIEW图像处理方法测量血液流速,客观地反映了血虚大鼠微循环的病理改变,表明显微医学图像分析技术在中医血虚证研究中具有良好的应用前景。

Objective：To observe changes of mesenteric microcirculation in rats with syndrome of blood deficiency,based on micro image analysis.Method： Acetyl phenyl hydrazine（APH） was subcutaneously injected on the back of rats to establish a blood deficiency model.The blood flow of mesenteric microcirculation was observed based on BI-2000 medical image analysis system,and the microcirculation image was acquired and analyzed through LabVIEW IMAQ Vision software,and the blood velocity was measured by tracing leukocyte.Result：①The color of mesenteric microcirculation in blood deficiency rats was dim,microvascular was deformed and its vascular wall was damaged.②The change of blood velocity of mesenteric microcirculation was matched with findings of modern research.③Blood flow of mesenteric microcirculation in blood deficiency rats showed a line flow state with particle and a aggregation flow state,and blood velocity was lower compared with common group（P0.05）.Conclusion： There exists vescular wall injury in rats with syndroms of blood deficiency,as well as blood stasis and exudation.Measurement of blood velocity by image ananlysis based on LabVIEW objectively can reflect pathological changes of mesenteric microcirculation in rats syndroms of with blood deficiency which shows that microscopic medical image analysis technique in terms of research traditional Chinese medicine syndrome of blood deficiency has a good application prospect.