柏叶汤中凉血止血药对脾胃虚寒出血大鼠血清TXB_2,6-keto-PGF_(1α)的影响

Effect of the Herbs for Removing Heat from Blood to Arrest Bleeding in Boye Decoction on TXB_2 and 6-keto-PGF_(1α) in Rats with Bleeding Caused by Cold of Insufficiency of Spleen-stomach

目的:研究柏叶汤方中凉血止血药对脾胃虚寒出血大鼠血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α)的影响。方法:SD大鼠随机分为正常组、模型组、柏叶汤组和柏叶汤去凉血止血药组,除正常组以外,均采用饮食失节法建立脾胃虚寒模型,共14 d。从造模第8天开始,模型组生理盐水ig,干预组给予柏叶汤(9 g.kg-1)和柏叶汤去凉血止血药(6 g.kg-1),ig,1次/d,连续7 d。除正常组外用乙醇灌胃法造成胃出血模型,采用酶联免疫法检测大鼠血清TXB2,6-keto-PGF1α的水平。结果:全方组能显著提高血清TXB2[(4.54±1.14)mg.L-1]而下调6-keto-PGF1α[(2.90±1.47)mg.L-1]的水平,与模型对照组[分别为(3.70±0.66,5.33±1.29)mg.L-1]及柏叶汤去凉血止血组比较差异有统计学意义,而柏叶汤去凉血止药组与模型对照组比较无统计学意义。结论:柏叶汤方可能是通过调节TXB2,6-keto-PGF1α水平促进凝血。

Objective：To study the effect of the herbs for removing heat from blood to arrest bleeding in Boye decoction on thromboxane B2（TXB2） and 6-keto-prostaglandin F1 alpha（6-keto-PGF1α） in rats with bleeding caused by cold of insufficiency of spleen-stomach.Method：The SD rats were randomly divided into 4 groups： normal control,model,Boye decoction and herbs for removing heat from blood to arrest bleeding in Boye decoction.The model of cold of insufficiency of spleen-stomach was made by intemperance of taking food for 14 days.Interventions started from the 8th day of modeling,the control group and the model group were ig given normal saline.Boye decoction group was given the decoction at a dose of 9 g·kg-1 daily.The group of herbs for removing heat from blood to arrest bleeding in Boye decoction was given at 6 g·kg-1 daily.All the interventions were for 7 days.The effect on TXB2 and 6-keto-PGF1α in SD rats was observed by using enzyme linked immunosorbent assay.Result：Boye decoction could significantly increase the level of TXB2 and decrease the level of 6-keto-PGF1α,compared with model group and the group of herbs for removing heat from blood to arrest bleeding in Boye decoction.The group of herbs for removing heat from blood to arrest bleeding in Boye decoction did not show significant difference compared with the model control.Conclusion：The main hemostasis mechanism of the herbs for removing heat from blood to arrest bleeding in Boye decoction is likely related the regulation of TXB2 and 6-keto-PGF1α.