白芍总苷对实验性变态反应性脑脊髓炎大鼠血清Th1型细胞因子的影响

Effect of Total Glucosides of Peony on Thl Cytokines in Experimental Allergic Encephalomyelitis Rats

目的:探讨白芍总苷(TGP)对实验性变态反应性脑脊髓炎(EAE)发病的治疗作用及其免疫学机制。方法:将50只大鼠随机分为正常对照组,EAE对照组及低,中,高剂量TGP治疗组,低,中,高剂量TGP治疗组大鼠造模后分别ig TGP 50,100,200 mg.kg-1.d-1,EAE对照组大鼠造模后ig等量生理盐水,观察各组的发病情况及血清中Th1型细胞因子γ干扰素(IFN-γ),肿瘤坏死因子α(TNF-α)水平的变化。结果:TGP各剂量治疗组的症状均较EAE对照组轻,发病潜伏期均较EAE对照组明显延长,高峰期症状评分均较EAE对照组明显降低;各TGP治疗组治疗后血清IFN-γ,TNF-α水平均显著低于EAE对照组。上述改变呈剂量依赖性。结论:TGP对EAE具有治疗作用,其治疗效果呈剂量依赖性;其治疗机制与抑制Th1型细胞因子(IFN-γ,TNF-α)产生有关。

Objective：To explore effects of total glucosides of peony（TGP） on experimental allergic encephalomyelitis（EAE） and the possible immunologic mechanisms.Method： Fifty female rats were randomly divided into five groups： the normal group,EAE model group and TGP low,medium,high dose,10 rats each group.TGP low,medium and high dose group were ig given 50,100,200 mg·kg-1·d-1,while the EAE model group was given equal dose of physiological salt water.The latent period and the maximal disease score of the rats and the level of Thl cytokines interferon gamma（IFN-γ）and tummor necrosis factor alpha（TNF-α） in blood serum were observed.Result： Each dose group of TGP had lighter symptoms than the EAE model group,the latent period was longer and the maximal disease score was lower than EAE model group.The level of TNF-α and IFN-γ in blood serum was lower than the EAE model group,which shoued a manner of dose-effect dependent.Conclusion： TGP has therapeutical effect on EAE,and the effect may be related to the decreased level of IFN-γ and TNF-α.