降脂平肝汤含药血清对HepG2细胞胰岛素抵抗的影响

Effects of Serum Containing Jiangzhi Pinggan Decoction on Insulin Resistance in HepG2 Cell Line

目的:探讨降脂平肝汤含药血清对HepG2细胞胰岛素抵抗的影响。方法:以浓度分别为5×10-5,5×10-6,5×10-7,5×10-8,5×10-9mol.L-1的胰岛素孵育HepG2细胞24 h,建立并筛选最佳HepG2胰岛素抵抗模型,分别以20%大鼠空白血清及5μmol.L-1罗格列酮为对照,采用细胞培养、糖原染色、葡萄糖摄取分析等方法检测20%降脂平肝汤含药血清对HepG2细胞胰岛素抵抗相应指标的变化。结果:以5×10-7mol.L-1的胰岛素成功建立HepG2胰岛素抵抗模型。与胰岛素抵抗模型组相比,降脂平肝汤含药血清组及罗格列酮组在基础状态下和胰岛素刺激状态下细胞的葡萄糖摄取以及糖原含量均明显增加,细胞糖原颗粒显著增多,差异具有统计学意义(P<0.05)。结论:降脂平肝汤含药血清能减轻HepG2细胞的胰岛素抵抗。

Objective：To observe the effect of serum containing Jiangzhi Pinggan decoction on insulin resistance（IR） in HepG2 cell line.Method： The model of IR in HepG2 cell line was established,and the serum of normal rats and rosiglitazone was colledted as the control,then the changes of IR related indexes in HepG2 cell line induced by Jiangzhi Pinggan decoction containing serum were detected with method of cell culture,PAS and glucose uptake analysis.Result： The model of IR in HepG2 cell line was established successfully,and compared with the model of IR,in Jiangzhi Pinggan decoction containing serum group and rosiglitazone group,the glucose uptake,glycogen content and glycogen granules in cells were elevated significantly both in the basic state and in insulin stimulate state,the differences were statistically significant（P0.05）.Conclusion： IR in HepG2 cell line could be alleviated with Jiangzhi Pinggan decoction containing serum.