高效液相色谱法同时测定忍冬中7种成分

Simultaneous determination of seven compounds in Lonicera japonica by high performance liquid chromatography

建立了在不同时间段内转换使用不同波长同时测定忍冬花和叶中7种化学成分(绿原酸、咖啡酸、芦丁、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C)的高效液相色谱分析方法,同时应用该方法分析了忍冬花、忍冬老叶和新叶中成分含量的差异。色谱柱为Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm);流动相为0.3%甲酸水溶液(A)和乙腈(B),梯度洗脱,流速1 mL/min;采用VWD紫外检测器转换波长(330 nm、350 nm)检测。应用所建立的方法测定忍冬新叶中绿原酸、木犀草苷含量分别为2.572%、1.498‰,均比药典中规定的含量高,有必要进一步的研究和开发利用。该方法准确、简便、灵敏度高,适用于忍冬中7种化学成分含量的同时测定和忍冬的质量控制及综合评价。

A high performance liquid chromatographic（HPLC） method was developed to determine the contents of seven compounds in Lonicera japonica and Folium Lonicerae with maximum wavelength conversion program,and analyze the content differences between them.The separation was performed on an Agilent Eclipse Plus C18 column（250 mm×4.6 mm,5 μm） operated at normal temperature with the gradient elution by two mobile phases of water（including 0.3%（v/v） formic acid）（A） and acetonitrile（B） at a flow rate of 1 mL/min.The maximum detection wavelength was set at 330 nm and 350 nm by conversion.The contents of chlorogenic acid and caffeic acid in Lonicera new leaves were 2.572% and 1.498‰ respectively,both higher than those cited in Chinese Pharmacopoeia,indicating that Lonicera new leaves have the necessity to be further studied and developed.This method is simple,rapid and highly sensitive.It is suitable for the simultaneous determination of the seven compounds and the quality control of Lonicera japonica.