前列腺素E_2对体外培养的新生大鼠心肌细胞的机能及超微结构的影响

The effect of prostaglandin E_2 on the function and ultrastructure of cultured new born rat heart muscle cells

本实验观察了PGE_2对体外培养的新生大鼠心肌细胞的机能及超微结构的影响。发现4ng/ml PGE_2可以立即引起细胞搏动频率明显减慢,作用6分钟对心肌细胞及微丝的形态结构没有明显影响;作用6小时,其微丝排列规则而整齐,与正常比较未见异常,但心肌细胞膜出现皱折,微足突变粗短甚至消失,微泡也消失。表明PGE2对心肌细胞的作用具有时间依赖性。

PGE_2 reduced pulsation in concentration of 4 ng/ml and pause in 40 ng/ml for cultured rat heart muscle cells. Membrane and microfilament changes slightly in cultured heart muscle cells exposed to PGE_2 4 ng/ml for 6 min. But membrane presented marked distortion in cultured heart muscle cells exposed to PGE_2 4 ng/ml for 6 hr. These data indicated that effect of PGE_2 is time-dependent.