单克隆抗体AU_(14-1)测定的抗原在人宫颈病变中的表达

Expression of the antigen detected by the monoclonal antibody AU_(14-1) in human cervical lesions

本研究应用抗小鼠宫颈癌(U_(14))单克隆抗体(AU_(14-1))对人宫颈癌和癌前病变进行免疫组织化学分析,以观察AU_(14-1)测定的抗原在人宫颈病变中表达的发生率和意义。所用材料为福尔马林固定和石蜡包埋的活检标本。切片经过氧化物酶抗过氧化物酶(PAP)染色。结果发现,宫颈鳞状细胞癌的单克隆抗体免疫组织化学染色的阳性率为20/20,腺癌为13/13,原位癌为15/21,非典型增生为2/20,慢性炎为2/24。提示U_(14)抗原的表达可能与宫颈的恶性转化有关。

In this article, monoclonal antibody to mouse uterine cervical carcinoma (U_(14)), AU_(14-1), was used for immunohistochemical analysis of premalignant and malignant lesions of the cervix to investigate the incidence and significance of the expression of the antigen detected by AU_(14-1) in human cervical lesions. Peroxidase-antiperoxidase (PAP) staining was performed on formalin-fixed, paraffin-embedded biopsy specimens. It was reported that positive staining with AU_(14-1) was detected in 20 of the 20 squamous cell carcinomas, 13 of the 13 adenocarcinomas, 15 of the 21 carcinomas in situ, 2 of the 24 anaplasia, and 2 of the 20 chronic cervicitis. It is suggested that the expression of the U_(14) antigen may be related to malignant transformation in the cervix.