摘要
从黑虎掌菌的子实体中分离纯化得到两种多糖SIPa和SIPb,考察了它们的红外光谱特征,通过高效凝胶渗透色谱法和气相色谱法测定其相对分子质量和单糖组成,用MTT比色法进行抗肿瘤活性评价.结果表明:SIPa和SIPb都具有典型的多糖红外吸收,SIPa的糖链以β型吡喃糖苷为主;SIPa和SIPb的重均相对分子质量分别为2.12×105和1.05×104;SIPa是一种葡聚糖,SIPb是一种杂多糖,含有岩藻糖、甘露糖、葡萄糖和半乳糖,摩尔比为8.93∶1∶4.25∶29.18;SIPa和SIPb对人肝癌细胞Hep G2和人卵巢癌细胞HO-8910都具有明显的抑制作用,且呈剂量依赖性.
Two kinds of polysaccharides,namely SIPa and SIPb,were obtained from the fruit body of Sarcodon imbricatus via isolation and purification.Then,the IR spectra of the products were investigated,and the relative molecular mass and monosaccharide composition of each product were determined by means of high-performance gel permeation chromatography and gas chromatography.Moreover,the antitumor activities of the two products were evaluated by means of the MTT method.The results show that(1) SIPa and SIPb both possess typical infrared absorptions of polysaccharides;(2) the sugar chain of SIPa is mainly β-configuration pyranoside;(3) the weight-average relative molecular mass of SIPa and SIPb are respectively 2.12×105 and 1.05×104;(4) SIPa is a kind of glucan while SIPb is a kind of heteroglycan containing fucose,mannose,glucose and galactose in a molar ratio of 8.93∶ 1∶ 4.25∶ 29.18;and(5) both SIPa and SIPb remarkably inhibit the growth of human liver cancer cell line Hep G2 and human ovarian cancer cell line HO-8910 in a dose-dependent manner.
出处
《华南理工大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2011年第12期110-114,共5页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省科技计划项目(2009B011300002)
关键词
黑虎掌菌
多糖
单糖组成
抗肿瘤活性
Sarcodon imbricatus
polysaccharides
monosaccharide composition
antitumor activity