儿童肺炎支原体感染者．Thl7／Treg的变化分析与临床意义

Changes and Clinical Significance of Thl7/Treg in Children with Mycoplasma Pneumoniae Infection

目的探讨外周血辅助性T细胞17型（helperTcelltype17，Thl7）和CD4^＋CD25^＋调节性T细胞（RegulatoryTcells,Treg）在肺炎支原体感染儿童体内的变化及临床意义。方法肺炎支原体（mycoplasmapneumoniae，MP）感染组32例．分为普通肺炎组18例、肺炎合并肺外症状组14例；健康对照组26名。常规分离外周血单个核细胞，流式细胞术检测各组外周血Thl7细胞、Treg的表达频率；ELISA方法检测血清细胞因子IL-6、IL-17水平。结果1．MP感染组外周血Thl7细胞频率高于正常对照组（P〈0．01），肺外症状组血Thl7细胞频率水平高于普通肺炎组（P〈O．01）；MP感染组外周血Treg细胞表达频率低于正常对照组（P〈0．01），肺外症状组血Treg细胞频率低于普通肺炎组（P〈0．01）。2．MP感染组血清IL．6、IL．17水平高于正常对照组（P〈0．01）；肺外症状组血清IL-6、IL-17水平高于普通肺炎组（P〈0．01）。结论在儿童MP感染者体内存在Treg与Thl7细胞频率明显改变，并伴有炎症性细胞因子水平升高．提示Treg与Thl7细胞的频率异常在儿童MP感染的发病机制中起着重要的作用。

Objective To study the change and the clinical significance of helper T cell type 17（Thl 7） and CD4＋CD25＋ regulatory T cells（Treg） in children suffering from mycoplasma pneumoniae（MP） infections. Methods 32 patients with MP infection were devided into mycoplasma pneumoniae pneumonia（MPP） group including 18 cases and MPP with lesion of exo-lung group including 14 cases.26 health children were treated as normal control group.The peripheral blood mononuclear cells were isolated,then frequnece of Thl7 and Treg cells were determined with flow cytometry.Serum contents of IL-6 and IL-17 were detected with ELISA.Results 1.The frequece of Thl7 cells of the patients with MP infection was obviously higher than that of the persons in normal control group（P 〈 0.01）,and MPP with lesion of exo-lung group was higher than MPP group（/＇ 〈 0.0 I）. The frequece of Treg cells of the patients with MP infection was significantly lower than that on normal control group（P 〈 0.01）,MPP with lesion of exo-lung group was obviously lower than MPP group（P 〈 0.01）.2.The serum levels of IL-6 and IL-17 in cases of MP infections were higher than those in normal control group（P 〈 0.0 1）,MPP with lesion of exo-lung group was higher than MPP group（P 〈 0.01）.Conclusion In vivo of children with MP infection,there were obvious changes of frequences of Thl7 and Treg cells accompanied with elevation of the serum levels,which suggests that the abnormal frequences of Th 17 and Treg cells play a key role in the pathogenic mechanism of MP infection in children.