附子对小鼠超氧化物岐化酶活性和丙二醛、NO含量的影响

Effects of Aconite on the Activity of SOD and Contents of MDA and NO in Mice

目的:研究附子不同的临床使用剂量对小鼠心脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)、一氧化氮(NO)含量的影响,探讨其对心脏的毒性机制方法:制备附子及与甘草配伍的水煎液,将小鼠随机分为9组,分别以附子15,45,75,105g的临床使用剂量按等效换算系数换算成小鼠给药卉剂量,给小鼠灌胃20d,测其心脏组织SOD活性及MDA、NO含量。结果:与对照组比较,单附子临床剂量45,75,105 g组小鼠心脏SOD活性明显增加,MDA含量显著降低(P<0.05)。甘草配伍后的各组与对照组比较,SOD含量有增加的趋势,但仅105 g组有明显差异(P<0.05);除15 g组外,其余各组MDA含量均显著降低(P<0.05);NO含量均呈下降趋势,但差异无统计学意义(P>0.05)。结论:大剂量使用附子对心脏的SOD活力、MDA和NO含量没有负面影响,相反能减轻小鼠心脏组织氧化反应。

Objective ： To study the effects of aconite with different doses on the activity of cardiac SOD and contents of MDA and NO in mice, and explore its cardiac toxicity mechanism. Method： The decoctions of aconite with or without the combination of licorice were prepared. The mice were randomly divided into 9 groups and given the decoctions with aconite doses of 15,45,75 and 105 g according to the equivalent clinical doses by gavage for 20 days. The activity of cardiac SOD, and MDA and NO content in mice were observed. Result： Compared with the control group, cardiac SOD content in the groups with aconite doses of 45,75 and 105 g was increased significantly while the MDA content was significantly decreased （ P 〈 0. 05 ）. The SOD content in the groups with aconite and licorice showed an increasing tendency while only the group with aconite dose of 105 g had significant difference compared with the control group （ P 〈 0. 05 ）. The MDA content was all in lower level except for the group with aconite dose of 15 g （ P 〈 0. 05 ）. Compared with the control group, the NO content of the groups was reduced with no significant difference （P 〉 0. 05）. Conclusion： High dose of aconite shows no negative effects on cardiac SOD, MDA and NO content. Furthermore,it can reduce cardiac oxidation in mice.