草豆蔻挥发油的小鼠急性毒性实验

Acute Toxicity of Volatile Oil from Alpinia katsumadai Hayata in Mice

目的:观察草豆蔻挥发油对小鼠急性毒性反应,评价其安全性大小。方法:给小鼠灌服和腹腔注射不同剂量浓度的草豆蔻挥发油,观察小鼠的活动和毒性反应,记录小鼠的死亡数,并用Bliss法计算半数致死量(LD_(50))。结果:草豆蔻挥发油对小鼠经口服及腹腔注射的急性毒性症状主要有:行动迟缓、异步态、心率加快、呼吸急促、连续性抽搐。小鼠口服给药的LD_(50)为237.8 g·kg^(-1)·d^(-1)95%可信限为277.9～203.4 g·kg^(-1)·d^(-1),腹腔给药的LD_(50)为157.9 g·kg^(-1)·d^(-1),95%可信限为176.9～140.9 g·kg^(-1)·d^(-1)。结论:草豆蔻挥发油毒性较低,为进一步开发应用提供依据。

Objective： To observe the acute toxicity of volatile oil from Alpinia katsumadai Hayata in mice and evaluate its safety. Method ： Different doses of volatile oil from Alpinia katsumadai Hayata were given to the mice by intragastric administration and intraperitonea[ injection. The movement and toxic symptoms were observed, and the number of dead mice was recorded. LD50 was calculated by Bliss. Result： Slowly action, abnormal walking, accelerated heart rate, breathlessness and consecutive convulsion were the main acute toxic symptoms and signs caused by volatile oil from Alpinia katsumadai Hayata. The LD50 of intragastric administration was 237. 8g·kg-1·d-1 with 95 % confidence interval of 277. 9-203.4g·kg-1·d-1. The LD50 of intraperitoneal injection was 157.9 g·kg-1·d-1 with 95% confidence interval of 176. 9-140.9g·kg-1·d-1. Conclusion： Volatile oil from Alpinia katsumadai Hayata is safe for clinical administration due to its low toxicity.