摘要
目的:建立磁性碳微球中5-氟尿嘧啶(5-fluorouracil,5-Fu)含量及包封率的HPLC测定法。方法:色谱柱为Diamon-sil^(TM)ODS-C_(18)柱(200 mm×4.6 mm,5μm),以甲醇-水(10:90)为流动相,流速1.0 ml·min^(-1),检测波长为260 nm,柱温为30℃,采用磁性分离法分离微球和游离药物,测定并计算药物含量和包封率。结果:5-Fu在1.0～300.0μg·ml^(-1)范围内线性关系良好(r=0.999 9),方法回收率为99.52%～100.12%,日内、日间RSD均小于1.0%,制备的微球平均包封率为(66.62±1.18)%,平均载药量为(221.69±3.99)μg·mg^(-1)。结论:本法简便,灵敏,准确,可用于5-Fu磁性碳微球的含量及包封率测定。
Objective： To establish an HPLC method for the determination of the content and entrapment efficiency of 5-fluorouracil （5-Fu）-loaded magnetic carbon microspheres. Method： Chromatographic separation was performed on a DiamonsilTM ODS-C18 reversed column （200 mm x4. 6 mm,5 μm） with methanol-water （ 10： 90） as the mobile phase at a flow rate of 1.0 ml.min-1. The wavelength of photodiode array detector （DAD） was set at 260 nm. The column temperature was 30 ℃. The free 5-Fu was separated from mierospheres by magnetic separation method and determined, and then the drug loading and entrapment efficiency were calculated. Result： The calibration curves showed good linearity over the range of 1.0-300. 0 μg·mg-1 （r =0. 999 9） for 5-Fu. The recovery was 99. 52% -100. 12%. The intra-day and inter-day RSDs were both less than 1.0%. The average encapsulation efficiency of the microspheres was （66. 62 ± 1. 18）% and the average drug loading was （221.69 ±3.99） μg·mg-1. Conclusion： The HPLC method is simple, sensitive and accurate, and can be used to determine the content and entrapment efficiency of 5-Fu-loaded magnetic carbon microspheres.
出处
《中国药师》
CAS
2011年第12期1759-1761,共3页
China Pharmacist
