转基因地被菊晚粉抗旱生理特性研究

Study on Physiological Traits of Drought Resistance for Transgenic Chrysanthemum Varieties Wanfen

对转太行菊OtMYB基因晚粉进行干旱胁迫和复水处理,以野生型晚粉和野生太行菊为对照,测定不同水分胁迫下叶片的叶绿素、可溶性蛋白、丙二醛、游离脯氨酸、可溶性糖的含量和膜透性,比较外源OtMYB基因表达对转基因晚粉抗旱性的影响。结果表明:随胁迫时间的延长,三者叶片中叶绿素含量均呈下降趋势,丙二醛含量、游离脯氨酸含量和相对电导率明显上升,但转基因晚粉各指标变化规律与太行菊相似,比野生型晚粉表现出更强的稳定性和恢复能力,表明OtMYB基因在转基因植株中成功表达,并提高了植株的抗旱性。转基因植株的可溶性蛋白和可溶性糖含量,表现出与OtMYB基因来源种太行菊相似的变化趋势,明显区别于野生型晚粉,推测OtMYB基因在干旱胁迫下能诱导转基因植株糖和蛋白的特异表达。

Transgenic chrysanthemum varieties Wanfen with OtMBY from Opisthopappus taihangensis,wide type Wanfen and Opisthopappus taihangensis were treated under water stress for 12 days ,then re-watered. In the experiments, chlorophyll, soluble protein, malondianldehyde （MDA） , proline, soluble sugar contents in leaves and relative permeability of cell membrane of leaves were determined. With the degree of drought stress increase, for all of the three,the content of chlorophyll in leaves decreased, while MDA, proline contents increased dramaticly. Compared with wild type Wanfen, However,the transgenic plants demonstrates greater stability and resistant,and showed similar physiological indicators variation to 0. taihangensis, which its OtMYB came from. This indicated that OtMYB gene had expressed in the transgenic plants and improved their drought resistance. Moreover, the trend of soluble protein and soluble sugar contents in transformed chrysanthemum was similar to that in 0. taihangensis, significantly different from that in wild type Wanfen, suggesting OtMYB gene probably could induce sugar and protein-specific expression under drought stress.