cDNA-SRAP标记在玉米氮素营养诊断上的应用初探

Preliminary Application of cDNA-SRAP Marker in Diagnosis of Maize Nitrogen Nutritional Status

为探讨对玉米氮素营养状况进行分子诊断的可行性,以大面积推广的玉米(Zea mays)杂交种郑单958为材料,用cDNA-SRAP标记的方法研究了玉米幼苗在不同氮素水平下基因表达的差异,对其中的一些差异表达片段进行了克隆和测序,并通过Real-time PCR鉴定了克隆的差异表达片段的真实性。结果表明:不同的SRAP标记引物对扩增效率不同,玉米基因表达受到氮素水平的严格调控,cDNA-SRAP标记的方法与Real-time PCR检测到的基因表达情况基本一致,基因差异表达片段N1、N9、N20、N30可作为玉米缺氮的分子诊断的候选靶标。因此cDNA-SRAP标记可以用来研究植物营养状况的分子诊断。

To investigate the feasibility of diagnosis for nitrogen nutritional status of maize in molecular level, the elite hybrid variety Zhengdan958 （Zea mays）was used to study the transcriptional difference of maize seedlings under different nitrogen levels by cDNA-SRAP markers. Several differentially expressed fragments were cloned and sequenced, and identified using Realtime PCR. The PCR amplification efficiency were various with different primer pairs. The nitrogen nutrition levels affected gene expression in maize. The results of cDNA-SRAP were basically con- sistent with those of Real-time PCR. Some fragments （ such as N1, N9, N20, N30） could be taken as the candidate targets of molecular diagnosis of nitrogen nutrition in maize, cDNA-SRAP markers is an efficient way to develop molecular diagnosis of plant nutrition.