与甜椒隐性细胞核雄性不育基因紧密连锁的SRAP分子标记的研究

SRAP Markers Linked to Recessive Genic Male Sterile Gene in Sweet Pepper(Capsicum annuum L.)

以甜椒隐性细胞核雄性不育两用系AB91为材料,采用混合集群分析法(BSA)构建了不育池与可育池。利用SRAP分子标记技术,筛选了225对SRAP引物组合及1 393对EcoRⅠ和MseⅠ引物组合,获得了与隐性核不育基因连锁的2个SRAP标记:E37M39、E44M93,片段长度约为200 bp和500 bp,与育性基因的遗传距离为6 cm和12 cm。

SRAP biotechnology combining with bulked segregation analysis（BSA） was used to identify the molecular markers linked to recessive genic male sterile gene in a dual purpose line AB91 of Capsicum annuum L.A total number of 256 SRAP primer combinations and 1 393 EcoRⅠ-MseⅠ primer combinations were used,and two SRAP markers E37M39 and E44M93 were identified to be linked to the recessive genic male sterile gene.The length was about 200 bp and 500 bp,and the genic distance was 6 cm and 12 cm respectively.