拮抗放线菌A02活性产物的分离和鉴别特性研究

The Characteristics for Separation and Identification of the Bioactive Metabolite of Antagonistic Actinomyces A02

为了明确利迪链霉菌A02抑菌活性物质的分离和鉴别特征,利用pH纸层析和纸电泳试验检测了其离子特性,有机溶剂萃取和硅胶薄层色谱分离测定了其极性,捷克八溶剂系统纸层析、紫外光谱扫描、官能团化学反应和蛋白酶敏感性试验分析了其化学类型。结果表明,菌株A02代谢活性产物在酸性和碱性溶液中呈电中性,但其极性较强,能被正丁醇高效萃取,乙醇∶氨水∶水(8∶1∶1,V/V)的溶剂组合对其薄层色谱分离效果较好;对蛋白酶K和胰蛋白酶不敏感,化学反应显示出不饱和烯键或炔键、糖苷键、氨基和醛基特性;结合纸层析谱型和紫外吸收图谱,判断其为四烯大环内酯类抗生素。此结果揭示了利迪链霉菌产生四烯大环内酯类抗生素的新功能。

In order to investigate the characteristics for separation and identification of the antifungal substance produced by antagonistic Streptomyce lydicus A02,the methods of pH paper chromatography,paper electrophoresis,organic solvent extraction,silica gel TLC,Doskochilova solvent system paper chromatography,ultraviolet spectrum scan,functional group and proteinase test were respectively used to determine the ion property,the polarity,the ultraviolet absorption character,the main functional groups and the sensitivity to proteinases of the bioactive substance of strain A02.The results showed that the antifungal substance were electrically neutral and highly polar in solutions,which enabled it to be efficiently extracted with n-butyl alcohol as the extractant and separated by TLC with ethanol ＋ aqua ammonia ＋ water（8∶ 1∶ 1,V/V） as the solvent combination.Its antifungal activity was not affected by proteinase K and trypsase.There were functional groups of unsaturated alkyl,saccharide or glycoside,amino acid and aldehyde in its structure according to the chemical reactions.Doskochilova paper chromatographic pattern and ultraviolet absorption spectrum of the antifungal substance were in accordance with the characters of tetar-alkene macrolide antibiotic.The combination of the information referred to above reveal a new biological function for S.lydicus to produce tetar-alkene macrolide antibiotics.