摘要
LRR类受体激酶RH4基因在花生黄曲霉敏感品种发育种子的种皮中上调表达。基于这类受体激酶多由于序列的变异导致抗性的变异,笔者将数据库中花生抗黄曲霉和敏感品种相似基因编码的蛋白进行序列比较分析,发现抗性品种与敏感品种的类似蛋白序列有很大差别。用荧光定量PCR方法对抗性品种KB153与敏感品种JH1012发育中不同时期的果实和部位进行差异表达分析,结果表明,果实发育的早期类受体蛋白激酶的大量表达可能是导致黄曲霉抗性的重要原因之一。
LRR type receptor like kinase（RLK） RHG is up-regulated in developing seed coat of sensitive strain relative to resistant strain to Aspergillus flavus.Based on the knowledgy that variation in sequences of LRR type RLK cause their function variety,we compared similar protein sequences encoded by ESTs from resistance and sensitive strains of Arachis hypogaea in public database,found that there were significant differences between protein sequences resistance and sensitive strains of Arachis hypogaea.We further conducted expression differential analysis on different tissues in different developing stages of both peanut Aspergillus flavus resistance and sensitive strains.The results indicate that up-regulation of expression of RLKis a possible reason of Aspergillus flavus resistance.
出处
《华北农学报》
CSCD
北大核心
2011年第4期198-201,共4页
Acta Agriculturae Boreali-Sinica
基金
与山东花生所的横向合作项目(HZY05013)
国家自然科学基因项目(NSFC307711361)
关键词
黄曲霉
花生
类受体蛋白激酶
抗性基因
Aspergillus flavus
Arachis hypogaea
Receptor-like protein kinase
Resistance gene
