自制HYD液对大鼠肝脏低温保存后生化功能影响的研究

Effect of cold prolonged storage of rat livers with self designed HYD-solution on hepatic biochemical functions

目的 研究自制HYD液对大鼠肝脏低温保存后生化功能的影响。方法 采用大鼠肝脏非循环离体灌注模型 ,比较HYD液、UW液和乳酸林格液 (LR液 )对大鼠肝脏 6、12、2 4、30、36h保存后生化功能的影响。结果 保存 12h的肝脏 ,其各项生化功能HYD组明显优于LR组 (P <0 .0 5 )。三磷酸腺苷 (ATP)、磷酸腺苷 (TAN)含量及Atkinson能荷 (AEC) ,HYD组略高于UW组 ,且保存 36h差异有显著性 (P <0 .0 5 )。线粒体H+ ATP酶水解活性及肝脏分泌胆汁量 ,HYD组略高于UW组 ,但差异无显著性 (P >0 .0 5 )。保存 30、36h后保存液 pH值 ,HYD组明显高于UW组 (P<0 .0 5 )。流出液酶学含量 ,HYD组与UW组差异无显著性 (P >0 .0 5 )。结论 HYD液保存鼠肝 12h效果明显优于LR液 ,保存 36h与UW液相当 ,在改善低温保存及再灌注肝脏能量代谢、预防细胞内酸中毒、分泌胆汁量及肝脏原位灌洗等方面 ,HYD液略优于UW液。

Objective To study the effect of cold prolonged storage of rat livers with self designed HYD solution on hepatic biochemical functions. Methods The preservation effect of HYD solution with that of UW solution and Lactate Ringer's solution (LR) was compared by using isolated perfused rat liver model. Results All the indices of hepatic biochemical functions in HYD group were better than in LR group for 12 h storage. The contents of ATP, TAN and AEC in HYD group were significantly higher than in UW group for 36 h storage ( P < 0.05 ).pH in HYD group was markedly higher than in UW group for 30 or 36 h storage( P < 0.05 ).The hydrolytic activities of H + ATP ase in hepatic mitochondria and bile production were mildly higher in HYD group than in UW group ( P > 0.05 ). No significant difference in AST,ALT and LDH in the effluent were found between HYD group and UW group ( P > 0.05 ). Conclusions The effect of 12 h storage of rat livers with HYD solution might be better than with LR solution. The effect of HYD solution is similar to that of UW solution within 36 h storage. However, HYD solution is slightly superior to UW solution in some aspects, such as improvement of energy metabolism during cold storage and reperfusion, prevention of intracellular acidosis, bile production and perfusion of liver in situ.