乙酰肝素酶和syndecan-1表达与脑胶质瘤恶性程度的关系

The relationship of the expression of heparanse and syndecan-1 in glioma tissues to the grades

目的:检测脑胶质瘤组织中乙酰肝素酶和syndecan-1的表达水平,探讨它们和肿瘤级别的关系及作用机制。方法:采用荧光实时定量聚合酶链式反应(RT-PCR)法检测乙酰肝素酶mRNA和syndecan-1 mRNA在40例脑胶质瘤组织和10例良性脑肿瘤组织中的表达水平,统计分析表达水平和肿瘤级别之间的关系。结果:在胶质瘤中,乙酰肝素酶的表达明显高于良性脑肿瘤(P<0.01),并且随着肿瘤的恶性程度增高,乙酰肝素酶的表达也明显增高(P<0.01),syndecan-1的表达明显低于良性脑肿瘤(P<0.01),并且随着肿瘤的恶性程度增高,syndecan-1的表达也明显降低(P<0.01)。乙酰肝素酶与syndecan-1在Ⅰ～Ⅱ级脑胶质瘤中表达均呈负相关关系(P<0.01),在Ⅲ～Ⅳ级脑胶质瘤中表达均呈负相关关系(P<0.01),在各级胶质瘤中表达相关性的总体分析均显示负相关关系(P<0.01)。结论:乙酰肝素酶在脑胶质瘤组织中高表达,syndecan-1在脑胶质瘤中低表达,而且表达水平和恶性程度密切相关,它们的检测可以作为脑胶质瘤恶性程度判断的参考,为探讨脑胶质瘤的生物学行为、预后及治疗提供新的思路。

Objective：To investigate the reltationship between the expression of heparanase and syndecan-1 gene mRNA and the pathological grade in human gliomas.Methods：The realtime polymerase chain reactions was empolyed to detect the expression level of heparanase and syndecan-1 gene mRNA in 40 cases of human gliomas and in 10 cases of benign brain tumors,which was used to evaluate tumor grade.Results：The expression level of heparanase mRNA in gliomas is higher than that in brain tumors（P0.01）,and rising with tumor grade.The expression level of syndecan-1 mRNA in gliomas is significant lower compared to that of benign brain tumors（P0.01）,and decresing with tumor grade.The expression level of heparanase mRNA is reversely correlated to the expression level of syndecan-1 mRNA in human gliomas（P0.01）.Conclusions：The heparanase and syndecan-1 gene expression maybe correlate to the oncogensis and progression of human gliomas,which can provide an effective way to diagnose and treat the human gliomas.