粒/巨噬细胞集落刺激因子对培养人牙周膜成纤维细胞生物学特性的影响

The Effects of GM-CSF on the Biological Activities of Cultured Human Periodontal Ligament fibroblasts

目的 :了解粒 /巨噬细胞集落刺激因子 (GM—CSF)对体外培养的人牙周膜成纤维细胞 (PDLFs)生物学活性的影响。方法 :利用体外培养人PDLFs的方法 ,观察不同浓度的GM—CSF对PDLFs的增殖、碱性磷酸酶活性、蛋白质合成量的影响。结果 :GM—CSF在较低浓度时即对PDLFs的增殖、碱性磷酸酶活性、蛋白质合成量有促进作用 ,它们的变化与浓度变化呈一定的相关性。结论

Aim:To investigate the effects of GM-CSF on the biological activities of cultured human periodontal ligament fibroblasts(PDLFs).Methods:Through applying in vitro cell culture method,to invsetigate the effects of GM-CSF on the changes of proliferation,alkaline phosphatase,protein content of PDLFs.Results:GM-CSF could promote these three biological activities at lower concentration,and the changes were related to the changes of concentration.Conclusion:The results suggested that GM-CSF might play a role in the process of alveolar bone remodeling through PDLFs.