基因组半定量PCR方法检测肿瘤组织的基因缺失

DETERMINATION OF GENE DELETION IN TUMOR TISSUE BY SEMI QUANTITATIVE POLYMERASE CHAIN REACTION

目的 用 PCR方法检测肿瘤组织中抑癌基因的缺失状况。方法 PCR半定量技术。结果 10对标本有 5对发现缺失 ,80 %与 Southern杂交结果相符合。结论 半定量 PCR技术可以替代 Southern杂交 ,成为检测基因缺失的一种便利手段。

Objective To determine the deletion of tumor suppressor gene in tumor tissue by PCR.Methods Semi quantitative PCR assay.Results 5/10 of the samples with partially loss of the SFY gene had been picked out by this method,and 80 % of them had been confirmed by Southern blot.Conclusion Semi quantitative PCR technique can replace Southern blot to be a convenient way to investigate gene deletion in tumor tissue.