枸杞多糖的分离纯化及其抗疲劳作用

Isolation and purification of lycium barbarum polysaccharides and its antifatigue effect

采用萃取、离子交换层析及凝胶层析从枸杞中获得枸杞多糖;将纯化的枸杞多糖(LBP-X)按5、10、20、50及100mg.kg-1.d-1分别灌喂小鼠,进行抗疲劳实验。结果表明,LBP-X能显著地增加小鼠肌糖原、肝糖原储备量,提高运动前、后血液乳酸脱氢酶总活力;降低小鼠剧烈运动后血尿素氮增加量,加快运动后血尿素氮的清除速率,以LBP-X10mg.kg-1.d-1剂量组效果最佳。提示LBP-X对提高负荷运动的适应能力,抗疲劳和加速消除疲劳具有十分明显的作用。但有其最适剂量,并非剂量越大效果越好。

A purified component of lycium barbarum polysaccharide (LBP X) was isolated from lycium barbarum L. by DEAE ion exchange cellulose and sephacryl gel chromatography. LBP X was tested on five different doses (5,10,20,50 and 100 mg·kg -1 ·d -1 ) in mice. The results showed that LBP X induced a remarkable adaptability to exercise load, enhanced resistance and accelerated elimination of fatigue. LBP X could enhance the storage of muscle and liver glycogen, increase the activity of LDH before and after swimming, decrease the increase of blood urea nitrogen (BUN) after strenuous exercise, and accelerate the clearance of BUN after exercise. The dosage of LBP X 10 mg·kg -1 ·d -1 was the best amount among the five tested doses.