黄瓜子叶节离体体细胞再生体系研究

Study on Cucumber Somatic Regeneration System via in vitro Cotyledonary Node Culture

以华北类型自交系L04-203为材料,通过对外植体类型、不定芽诱导培养基、芽伸长培养基等进行研究,建立了以子叶节为外植体通过器官发生方式的黄瓜离体体细胞再生体系。研究表明:附带短下胚轴子叶节为最适外植体;BA/NAA比例对愈伤形成和再生频率有直接影响,NAA为零或低浓度时,在外植体切口处直接形成再生芽,随着NAA浓度增加,切口处愈伤量增大,最佳芽诱导培养基为MS+BA/NAA(2/0.2 mg/L),每外植体出芽数可达3.8;芽伸长培养基为MS+BA 0.5 mg/L。该体系可用于相关转基因研究。

A Northern China cucumber inbred line L04-203 was used as plant material. Through systematic study on explant type,adventitious shoot induction medium and shoot elongation medium, an efficient cucumber so- matic regeneration system was established. The results indicated that, cotyledonary node with a little bit of hypocotyl was the best explants. Ratio of BA/NAA in the medium has a remarkable influence on regeneration rate and callus induction. Shoots were directly initiated in the incision when NAA concentration was 0 or very low, callus in the incision increased as NAA concentration increased. The optimum induction medium was MS ＋ BA/NAA（2/0.2 mg/L）. 3.8 shoots can be obtained per explant. The optimum shoot elongation medium was MS ＋ BA 0.5 mg/L.