摘要
分别以相同类型甜瓜品种东方蜜1号、雪里红和翠蜜为对照,对甬甜4号和甬甜5号甜瓜进行RAPD指纹图谱构建。在筛选的30个RAPD引物中,4个引物S304、S307、S327和S334稳定扩增出5个多态性位点,以操作简单的RAPD分子标记构建的指纹图谱,图谱出现概率为1/15120,能够有效区分5份材料,起到品种鉴定与保护作用。并在最后讨论了RAPD分子标记技术的可靠性。
The fingerprinting of Yongtian No. 4 and Yongtian No. 5 melon eultivars were established compared with the similar melon types of Dongfangmi No. 1, Xuelihong and Cuimi using RAPD molecular markers. Four markers, S304, S307, S327 and S334, were screened from 30 RAPD primers, which generated five polymorphic loci. Five similar melon cuhivars would be identified by the fingerprinting results with high resolution（1/15120）. And finally, the reproducibility of RAPD analysis among laboratories was also discussed.
出处
《华北农学报》
CSCD
北大核心
2011年第B12期34-36,共3页
Acta Agriculturae Boreali-Sinica
基金
宁波市国际合作项目(2010D10011)
宁波市农科院院长基金(10-05
11-08)
