干预协同刺激分子CD86对生殖道沙眼衣原体感染小鼠妊娠结局的影响

Effect of CD86 costimulatory signal blockade on the pregnancy outcome at early stage of gestation with vaginal chlamycial trachonatis infection

目的了解小鼠感染沙眼衣原体后的妊娠结局,以及CD86 mAb干预治疗后对妊娠结局的影响及与体内Th1型、Th2型细胞因子水平的关系。方法建立小鼠沙眼衣原体生殖道感染模型,合笼受孕后为模型组,受孕后孕4 d腹腔注射CD86 mAb100μg作为治疗组,将未受衣原体感染孕鼠作为对照组。孕12 d用酶联免疫法检测各组小鼠血清中Th1型(IFN-γ)和Th2型(IL-4)细胞因子的表达水平。结果①模型组Th1型细胞因子(IFN-γ)上升,较对照组和治疗组差异有统计学意义(P<0.05),②模型组胚胎吸收率升高,较对照组和治疗组差异有统计学意义(P<0.05)。③3组的受孕情况、胚重、孕期体质量增加量差异无统计学意义。结论小鼠感染沙眼衣原体后体内Th1细胞因子IFN-γ增加,形成Th1型偏倚,增加了胚胎吸收率;CD86 mAb干预可降低Th1型偏倚程度,降低小鼠胚胎吸收率。

[Objective] To explore the effects of CD86 antibody on the pregnancy outcomes, as of chlamydial traehomatis （CT） infection and administration well as Th-1 and Th-2 type eytokines. [Methods] The genital infection pregnant mouse model was established by inoculated with CT transvaginally followed by mating with male mice, which is considered as test group. The CT infected pregnant mice were administrated with CD86 mAb at day 4 after pregnancy. The pregnant mice without CT infection were set as control group. The level of sera eytokines Thl （IFN-30 and Th2 （IL-4） was detected by ELISA at day 12 of gestation. [Re- suits] Infection of CT increased Th-1 eytokine （IFN-3~） and embryo resorption of the pregnant mice, which was restored by administrated with CD86 mAb. No significant difference in failure rate of pregnancy, embryo weight and maternal weight increment was observed within the groups. [Conclusion] CT infection of the geni- tal tract is associated with Th-1 type immune bias and the embryo resorption rate. The CD86mAb reduced the embryo resorption rate of pregnancy and redress Th-I type immune bias.