大鼠心肌缺血再灌注损伤模型的制备及改进

Preparation and Improvement of Rat Model of Myocardial Ischemia Reperfusion Injury

目的总结并改进大鼠心肌缺血再灌注损伤(myocardial ischemia reperfusion injury,MIRI)模型的制备方法。方法将SPF级成年雄性SD大鼠60只,随机分为3组,每组20只。分别采用切断第4肋法,切断第2、3、4肋骨牵拉固定结扎线法,不切断肋骨使用小型扩胸器及双移液器枪头套叠代替打结法制备MIRI模型。结果 3种方法模型制备成功率分别为45%、50%、70%,成功率比较差异无统计学意义(P>0.05)。3种方法模型制备时间分别为(7.5±1.2)min、(9.0±1.9)min、(5.9±0.8)min,3组比较差异有统计学意义(P<0.05),方法三组与方法一、二组比较,差异均有统计学意义(P<0.05)。3组大鼠模型制备成功者再灌注后CK-MB值(1215±223)U/L与缺血时CK-MB值(1654±313)U/L比较,差异具有统计学意义(P<0.05)。结论不切断肋骨,使用小型扩胸器及双移液器枪头套叠代替打结法制备MIRI模型是目前较优良的模型制备方法。

Objective To summarize and improve the method of making rat myocardial ischemia and reperfusion injury（MIRI） model.Methods 60 SPF degree adult male SD rats were randomly divided into three groups（20 in each）.The three methods were used to manufacture the MIRI model： cutting off the fourth rib;cutting off the second,third and fourth ribs,pulling and fixing ligation line;if there was no need to cut off the ribs,the small breast expander and the pipettor spear were used instead of knot method.Results The success rate of the three methods were 45%,50% and 70%,respectively with significant difference（P〉0.05）.Making of the three models needed（7.5±1.2）min,（9.0±1.9）min and（5.9±0.8）min,respectively with significant difference（P〈0.05）,and methods three group compared with methods one,two group with statiatical difference（P〈0.01）. The index of CK-MB after reperfusion（1215±223）was different form that during ischemia（1665±313）（P〈0.05）.Conclusion There is no need to cut off the ribs,the small breast expander and the pipettor spear instead of knot method without cutting off ribs is preferred in manufacturing MIRI model.