摘要
目的:采用N-甲基-N-硝基-N-亚硝基胍(MNNG)和40%乙醇灌胃建立大鼠慢性萎缩性胃炎(CAG)模型,同时采用破气苦降,饥饱失常和疲劳的方法进一步建立CAG脾气虚证大鼠模型。观察CAG脾气虚证大鼠不同时期病理形态学的改变。方法:以170μg/mL的MNNG10mL/kg灌胃,150μg/mL的MNNG溶液给大鼠自由饮用,40%乙醇,2mL/只,每周2次,在此基础上第5周采用破气苦降,饥饱失常和疲劳的方法建立CAG脾气虚证大鼠模型,连续10周。每2周随机处死CAG脾气虚证大鼠2只进行光镜观察。结果:随着造模时间的延长动物胃部病变的进程是从正常胃黏膜-慢性胃炎-萎缩性胃炎-肠上皮化生-不典型增生-胃癌前病变。结论:采用MNNG和40%乙醇灌胃以及破气苦降,饥饱失常和疲劳的方法可成功建立慢性萎缩性胃炎脾气虚证模型。
Objective:Chronic atrophic gastritis rat model was induced by intragastric administration of MNNG and 40% ethanol. Furthermore, spleen deficiency syndrome of the CAG model was established by irregular feeding and overstrain. Pathological changes in different stages were observed. Methods :Intragastric administration of MNNG with the dose of 10mL/kg( 170μg/mL)was given and MNNG (150μg/mL) was for drinking. 40% ethanol was injected twice a week with the dose of 2mL for every rat. Besides, in the 5th week, spleen deficiency model was established by irregular feeding and overstrain continuously lasting 10 weeks. Every 2 weeks, two model rats were put to death randomly for light mi- croscope observation. Results: With the time passing by, the development of the CAG was as following:normal gastric mucosa - chronic gastritis - atrophic gastritis - intestinal metaplasia - atypical hyperplasia - gastric precancerous lesion. Conclusion:MNNG and 40% ethanol with irregular feeding and overstrain can successfully establish the spleen deficiency syndrome of chronic atrophic gastritis model.
出处
《中华中医药学刊》
CAS
2012年第2期231-233,I0001,I0002,共5页
Chinese Archives of Traditional Chinese Medicine
基金
江苏省科技厅"科技基础实施建设计划"专项(BM2009903)
关键词
慢性萎缩性胃炎脾气虚证模型
不同时期病理形态学的改变
spleen deficiency syndrome of chronic atrophic gastritis model
pathological changes in different stages