NR1基因敲除对小鼠前额叶脑区LTP的影响

Long-term potentiation of prefrontal cortex in NRl knockout mice

利用前脑特异性NR1基因敲除小鼠,采用离体脑片场电位技术,研究了NR1亚基在前额叶脑区突触可塑性中的作用.刺激强度一反应(input-output curve)和双脉冲抑制反应(pairedpulse depression,PPD)的结果表明,与同窝对照组小鼠相比,NR1基因敲除小鼠前额叶脑区的基本突触传递无明显变化.采用高频刺激(100 Hz,1 000 ms×2,间隔30 s)在小鼠的前额叶脑区诱导长时程增强(long-term potentiation,LTP),与对照组小鼠相比,NR1基因敲除小鼠前额叶脑区的LTP明显受损.以上数据提示,NR1亚基在前额叶脑区LTP的诱导中起着重要的作用.

This study investigated the prefrontal synaptic plasticity of forebrainspecific NR1 knockout mice by using the vitro field potential recording technique. The results from the inputoutput and paired pulse depression curves suggest that the basal synaptic transmission is normal in the prefrontal cortex of NR1 knockout mice. Prefrontal longterm potentiation （LTP） induced by high frequency stimulation （two trains of 100 Hz with 1 s duration and 30 s interval） was abolished in slices of NR1 knockout mice. These results indicate that NR1 is critical in the induction of longterm potentiation in prefrontal cortex.