脉络丛细胞微囊脑内移植前后的物理及生化性能研究

Research on the Physical and Biological Properties of Choroid Plexus Cells Micro-capsule Before and After Transplantation in the Brain

通过测定脉络丛细胞海藻酸盐微囊在大鼠脑内移植前及移植后的物理及生化性能变化,以探讨其应用于移植治疗神经系统疾病的可行性.用海藻酸盐多聚鸟氨酸微囊包裹猪脉络膜细胞,移植至大鼠黑质-纹状体通道,移植前、移植后4个月及6个月分别测定微囊的大小、形态及细胞的活力、分泌蛋白质及神经营养因子的能力、蛋白质组学的变化.脉络膜细胞微囊在移植前、后大小、细胞活力、蛋白质组学分析、分泌蛋白质及神经营养因子的能力无显著变化.海藻酸盐-多聚鸟氨酸CP微囊能有效地防止脉络膜细胞被受体免疫系统所攻击,使得它们能在大鼠的大脑存活6个月以上并不引起不良作用.

To investigate the physical and chemical changes of alginate capsuled choroid plexus before and after transplantation in the rat brain, poly-ornithine alginate microencapsulated porcine choroid cells were transplanted into rat substantia nigra-striatum channel. The size, shape and cellular vitality of the microcap- sules, the ability of protein and neurotrophic factors secretion and changes in proteomics before transplanta- tion, 4 and 6 months after transplantation were measured. In transplanted microencapsulated choroid cells, the size, cell viability, proteomics analysis of secreted proteins and the ability of neurotrophic factors did not change significantly. The alginate-poly-ornithine microcapsules CP can effectively prevent them from being attacked by the immune system receptors, so they can survive in the rat brain and does not cause adverse ef- fects in more than six months.