摘要
利用纤维素酶从桑葚中提取桑葚多糖,通过单因素实验和L9(34)正交实验研究酶用量、酶解时间、酶解温度对桑葚多糖提取率的影响。实验结果表明:纤维素酶能够显著提高桑椹多糖的提取率,并且提取温度是最重要的影响因素,其次是酶解时间,酶用量在此实验范围内对测定结果的影响最小,提取的最佳工艺条件为:酶解温度45℃,酶解时间150 min,酶用量4.0 mL。
extraction of polysaccharide from mulberry fruit by cellulose enzyme was studied in this paper ~ Single factor and orthogonal design methods L9 （ 34 ） were applied to analyze the effects of the factors such as the concentraction, time and temperature of the enzyme amount of mulberry polysaccharide. The results indicated that the temperature would significantly affected the extraction percent of mulberry polysaecharide. The optimum temperature was 45 ℃, the extracting time 150 min, and the enzyme amount 4.0 mL.
出处
《中国野生植物资源》
2012年第1期30-32,共3页
Chinese Wild Plant Resources
关键词
桑葚
多糖
纤维素酶
提取
mulberry fruit
polysaccharide
cellulose enzyme
extraction
