摘要
目的观察白细胞介素13(IL-13)对急性缺血再灌注肾损伤大鼠IL-6表达的影响。方法将Wistar雄性大鼠37只随机分为假手术组、I/R组、C组、T-S组和T-L组。阻断大鼠双侧肾脏血流45 min,再灌注24 h建立急性肾缺血再灌注模型;T-S组和T-L组于阻断血流后分别从双侧肾动脉开口注射入鼠重组白细胞介素13 0.5μg/kg和1.5μg/kg;C组以生理盐水代替。检测各组大鼠IL-6血清水平和肾脏表达情况以及肾功能和肾脏病理变化。结果 T-L组肾脏IL-6基因和蛋白表达明显减少,IL-6血清水平也明显下降;T-L组肾功能障碍、肾组织病理变化明显减轻,肾小管损害评分明显减少;血清IL-6水平和肾组织IL-6 mRNA与BUN、Cr均呈正相关。结论 IL-13能有效抑制大鼠急性肾缺血再灌注损伤IL-6的表达和保护肾功能。
Objective It is to observe the effects of IL -13 on expression of IL- 6 in acute renal ischemia/reperfusion in- jury in rats. Methods Thirty-seven male Wistar rats were randomly divided into 5 groups： sham group, I/R group, C group, T - S group and T - L group. Models of acute renal ischemic/reperfusion injury were established by blocking up kidneyg blood flow in both side for 45 rain and reperfusion for 24h in the rats. Rm - IL - 13 was injected into the renal- arteries through the abdominal aorta in T - S group and T - L group（ T - S 0.5 μg/kg body weight, T - L 1.5μg/kg body weight） ,normal saline instead of rm - IL - 13 was injected into the renal arteries through the abdominal aorta in control group. The serum level of IL - 6 and the renal expression of IL - 6 were determined in each group at 24 h post-ischemia. In addition, BUN, Cr and renal his- tology were also measured. Results The serum level of IL - 6 gene expression and protein production of IL - 6 in kidney decreased markedly in T - L group. Renal function and histology were significantly improved in T - L group, renal injury scores decreased significantly too. A positive correlation was found between the serum level of IL - 6, gene expression IL - 6 in kid- ney and BUN, SCr. Conclusion IL - 13 can inhibit the expression of IL - 6 and improve function and histology of kidney in rats with acute renal ischemia/reperfusion injury.
出处
《现代中西医结合杂志》
CAS
2012年第7期707-709,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
