松香药材中松香酸的含量测定方法

Determination of abietic acid in colophony

目的建立松香药材中松香酸含量的测定方法。方法用RP-HPLC测定,用Diamonsil C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-1%醋酸铵溶液(85∶15),流速为1.0ml/min,紫外检测器,检测波长241nm,柱温30℃,检测松香药材中松香酸的含量。结果松香酸在10～250μg/ml的浓度范围内与峰面积呈良好的线性关系Y=40.15X+32.63,(r=0.9999),平均回收率为99.3%(RSD=0.42%)。结论所用方法简便可行,灵敏度高,重复性好,可用于测定松香药材中松香酸的含量。

Objective To establish the determination method of abietic acid in colophony.Methods RP-HPLC analysis was carried out with Diamonsil C18（250 mm×4.6 mm,5μm） column and methanol-1% ammonium acetate（85∶15） as the mobile phase at the flow rate of 1.0ml/min,the detection wavelength was 241nm and the column temperature was 30℃.Results The linear relationship of abietic acid in the range of 10~250μg/ml（r=0.9999） and the average recovery was 99.3%（RSD=0.42%）.Conclusions The method is convenient and sensitive,and can be used for the determination of abietic acid in colophony.