油茶中茶皂素的膜分离-大孔树脂联用技术的研究

Study on Theasaponin of Camellia Oleiferal Abel.by Membrane Separation Combined with Macroporous Resin Adsorption

采用陶瓷膜与大孔树脂吸附技术联用分离纯化油茶饼粕提取液中的茶皂素。将油茶饼粕提取液先过陶瓷膜,以膜通量、油茶皂素转移率与除杂率为主要指标进行优化选择,再将陶瓷膜透过液进行大孔树脂吸附,以茶皂素的收率、纯度为考察指标进行工艺优化。结果表明:优化的纯化工艺为油茶饼粕提取液过0.05μm的陶瓷膜,料液质量分数为1%,操作压力为0.15 MPa;陶瓷膜透过液上AB-8大孔树脂柱,先以水洗至无色,再以0.2%NaOH溶液洗至流出液颜色较淡,水洗至中性,再以60%乙醇洗脱,60%乙醇部分为茶皂素部位,总茶皂素质量分数大于95%。采用膜分离-大孔树脂联用技术得到的茶皂素不仅纯度高、颜色淡,且该技术生产成本低,污染小,可以成为工业上生产茶皂素产品的一种新技术。

Theasaponin was purified from oil-tea-cake extract by the technique of ceramic membrane separation combined with macroporous resin adsorption. The conditions such as membrane flux, saponins transfer rate and impurity removal rate in ceramic membrane separation step were optimized. Then the conditions such as yield and purity of theasaponin in maeroporous resin adsorption step were optimized. The results showed that the best purification process was as follows：making oil-tea-cake extract to get through ceramic membrane,pore size is 0.05 μm,operating pressure is 0. 15 MPa, solution concentration is 1% ;then the extracts were adsorbed by AB-8 macroporous resin and eluted successively by water, 0.2 % NaOH to weak color, water to neutral and 60 % ethanol to obtain saponin fraction, the content of total saponin is over 95 %. The theasaponins got by the technique of ceramic membrane separation combined with macroporous resin adsorption have high purity and light color. This technique with low production cost and less pollution can be used in producing theasaponins industrially.