姜黄素对HL60细胞PI3K/AKT信号传导通路的影响

Effects of curcumin on PI3K/AKT signaling pathway in HL60 Cells

目的研究姜黄素诱导白血病HL-60细胞凋亡与PI3K/AKT信号传导通路的相关性,为AML的靶向治疗提供新思路。方法 MTT实验检测姜黄素对HL-60细胞增殖的抑制作用;经姜黄素处理后,用倒置显微镜进行细胞形态学观察,并用Western blot检测CAV-1、AKT、p-AKT蛋白水平。结果 MTT实验证明了姜黄素对HL60细胞增殖具有抑制作用,且随作用浓度的增强而增强;姜黄素作用HL-60细胞,用倒置显微镜观察细胞出现了凋亡形态学改变;并且Western blot检测显示CAV-1蛋白水平增高,而AKT、p-AKT蛋白水平降低。结论姜黄素可通过上调HL-60细胞中CAV-1的表达,而抑制PI3K/AKT信号通路,进而抑制HL-60细胞增殖并促进其凋亡。

Objective To investigate the effects of curcumin on the PI3K/AKT signaling pathway in HL60 cells and the apoptosis of ilL60 cells. Methods The inhibitory effects of curcumin on the proliferation of ilL60 ceils were determined by MTT assay. After being treated by curcumin, the morphological and structural changes of the cells were observed by inverted microscope. The levels of CAV-1, AKT and p-AKT protein were measured by Western blotl Results MTT assay revealed that curcumin has growth-inhibiting effects on I-IL60 cells, and the effects were en- haneed with the increase of concentration. Apoptotic morphology changes were observed by inverted microscope. The level of CAV-1 protein was increased, while the levels of AKT and p-AKT protein were reduced. Conclusion Curcumin can inhibit PI3K/AKT signaling pathway by upregulating the expression of CAV-1, resulting in inhibiting cell proliferation and promoting apoptosis in HL60 cells.