PCR-DGGE技术分析合生素对肉仔鸡盲肠菌群结构的影响

Influence of symbiotics on the bacterial community in the cecal contents of broilers analyzed by PCR-DGGE

试验研究合生素对肉仔鸡盲肠微生物区系的影响。选择1日龄肉仔鸡450只,随机分成5个处理,分别饲喂基础日粮、基础日粮+抗生素、基础日粮+益生素、基础日粮+益生元、基础日粮+合生素,每个处理3个重复,每个重复30只鸡。在21,42日龄每个重复选择2只鸡,无菌采集盲肠内容物,提取细菌基因组总DNA,PCR扩增16s rDNA V3区,扩增产物经变性梯度凝胶电泳(DGGE)后分析细菌群落结构的变化。结果表明,在214,2日龄,日粮添加合生素处理组试验鸡盲肠DGGE条带数均高于其他处理组,合生素组试验鸡盲肠内容物菌群多样性高于其他试验组。序列分析发现肉仔鸡盲肠中特异条带主要来源于不可培养的拟杆菌属、普雷沃氏菌属、梭菌目细菌和其他大量种属关系未知的细菌。

The influence of synbiotics on the predominant bacteria community in the cecal content of broilers was evaluated.Four hundred and fifty one-year-old broilers were randomly divided into five treatments of basic diets and basic diets supplemented with antibiotics,probiotics,prebioticts,synbioticts,respectively.On day 21 and 42,two chickens of each replicate were randomly selected and killed,the cecal contents were sterilely collected,and total cellular DNA from the cecal digesta were extracted and purified for PCR-DGGE,then diversity of microbiota was analyzed after DGGE.The results showed the chickens fed with synbiotics showed a higher bacterial diversity and more DGGE bands compared with other treatments on day 21 and 42.The specificity bands of cecal were from uncultured Bacteroidales,uncultured Prevotellaceae,uncultured Clostridiales and plenty of uncultured bacterial without classification.