苹果果实中阿魏酸和儿茶素的毛细管电泳分离测定研究

Study on determination of ferulic acid and catechin in apple fruits by capillary electrophoresis

利用毛细管电泳法对苹果中阿魏酸和儿茶素做了定性定量分析。通过分离条件的优化,确定了分离苹果中阿魏酸和儿茶素的最佳缓冲液为30mmol/L硼砂-硼酸+30%乙腈+50mmol/L SDS,pH=8,这两种酚类物质在10min内能较好地得到分离。得到儿茶素的峰面积和迁移时间的相对标准偏差(RSD)分别为2.39%和2.92%,阿魏酸的峰面积和迁移时间的RSD分别为3.41%和5.12%;儿茶素的回收率在90%～102%之间,阿魏酸的回收率在87%～105%之间。该方法简单、准确,可作为苹果果实品质测定的一种优良方法。

Ferulic acid and catechin in apple made a qualitative and quantitative analysis by capillary electrophoresis.The best buffer to determine the separation of ferulic acid and catechin in apple is 30 mmol/L borax-boric acid ＋30% acetonitrile ＋50 mmol/L SDS,pH8,both of them are well separated within 10 minutes.The RSDs of getting the peak area and migration times are 2.39% and 2.92%,and 3.41% and 5.12% respectively.This method measured made the recovery of catechin in 90% to 102%,recovery of ferulic acid in 87% to 105%.This method can be used as a simple and good determination of apple fruits quality.