全蝎醇提物与丙戊酸干预氯化锂-匹罗卡品慢性点燃模型大鼠癫痫发作的疗效比较

Effects of Ethanol Extracts of Scorpion and Valproic Acid in the epileptic rat model induced by Lithium Chloride-Pilocarpine:A comparative study

目的探讨全蝎醇提物(Ethanol Extracts of Scorpion,EES)与丙戊酸(Valproic Acid,VPA)干预氯化锂-匹罗卡品(Lithium Chloride-Pilocarpine,Licl-Pilo)慢性点燃模型大鼠癫痫发作的疗效。方法 186只成年雄性大鼠除6只设为正常对照组外,其余均建立Licl-Pilo慢性点燃大鼠模型,造模成功后分为模型组、VPA干预组、低EES剂量干预组(EESL干预组)、中EES剂量干预组(EESM干预组)和高EES剂量干预组(EESH干预组)各36只。正常对照组、模型组分别灌胃给予等容积的生理盐水,VPA干预组灌胃给予VPA溶液[浓度为120 mg/(kg.d)],EESL干预组、EESM干预组、EESH干预组灌胃给予EES溶液[浓度分别为290 mg/(kg.d)、580 mg/(kg.d)及1160 mg/(kg.d)],观察30天内致痫大鼠慢性癫痫自发性发作(spontaneous seizures,SRS)、发作级别、发作次数,并进行组间比较。结果 Licl-Pilo慢性点燃模型鼠的造模成功率为85.65%,各实验组SRS发生频率5～15次/周。治疗后,EESM干预组、EESH干预组和VPA干预组癫痫大鼠痫性发作级别及SRS发作次数与模型组比较差异有统计学意义(P<0.05);EESL干预组治疗效果不明显,与模型组比较差异无统计学意义(P>0.05)。结论一定剂量的EES能显著降低癫痫大鼠的自发性发作,且与疗效呈明显的量效关系,即剂量越大疗效越好,该结果为EES的抗癫痫作用提供了行为学依据。

A comparative study on the antiepileptic effects of Ethanol Extracts of Scorpion（EES） and Valproic Acid（VPA） in epileptic rat model induced by Lithium Chloride-Pilocarpine（Licl-Pilo）. Of 186 adult male SD rats,6 served as normal controls（control group）,the others were injected with Licl-Pilo as the epileptic rat model.The model rats were randomly assigned to the Licl-Pilo group（n=36）,the VPA group（n=36）,the low-dose EES group（EESL,n=36）,the middle-dose EES group（EESM,n=36）,and the high-dose EES group（EESH,n=36）.Rats in control and Licl-Pilo group were intragastrically administrated with isometric Sodium Chloride.The other model rats were intragastrically administrated with VPA 120 mg/（kg·d）,EES 290 mg/（kg·d）,EES 580 mg/（kg·d）,and EES 1160 mg/（kg·d） respectively.The model rats were observed for 30 days on the severity and frequency of chronic spontaneous seizures（SRS） which were also compared among groups. The success rate of the epileptic rat model was 85.65%.The mean incidence of SRS in all model rats ranged from 5 to 15 times per week.After treatment,the severity and frequency of SRS were significantly different between the Licl-Pilo,EESM,EESH and VPA group（P 0.05）.No significant difference was observed between the EESL and Licl-Pilo group（P 0.05）. EES can significantly reduce the incidence of SRS.There is an obvious dose-effect relation between EES and SRS.Those results provide behavioral evidences for the antiepileptic effect of EES.