实验考察柴胡与黄芩配伍前后柴胡皂苷a、d的溶出性差异

The experimental study on dissolution difference of saikosaponin a,d between the before and after bupleurum compatibility with the skullcap

目的通过实验考察柴胡黄芩配伍前后对柴胡药效部位柴胡皂苷溶出的影响。方法采用梯度高效液相色谱法建立测定柴胡皂苷a、d含量的方法,以相同条件分别提取柴胡药材和柴胡-黄芩药对,实验比较药对配伍前后柴胡皂苷a、d的溶出差异。结果药对中柴胡皂苷的含量明显高于柴胡药材。结论柴胡-黄芩药对的配伍,能显著提高药效成分柴胡皂苷的溶出率,临床常将二者配伍应用是合理的、有利于药效的提高。

Objective To study the effects on dissolution of bupleurum active parts saikosaponin between the before and af- ter bupleurum compatibility with the skullcap through experiment. Methods Gradient HPLC method was established for the de- termination of saikosaponin a,d,using the same conditions to extract herbs bupleurum and bupleurum -skullcap drug pairs re- spectively,and to compare the dissolution differences of saikosaponin a, d between the before and after compatibility. Results The contents of saikosaponin in bupleurum - skullcap drug pairs were higher than the herbs bupleurum. Conclusion The com- patibility of bupleurum - skullcap drug pairs can significantly improve the dissolution rate of saikosaponin. The application of com- patibility of the two drugs in clinical was reasonable and conducive to the improvement of efficacy.