高效液相色谱法同时对金银花中5种有效成分的含量测定

Simultaneous determination of five main components in lonicera japonica thunb by HPLC

目的建立HPLC法同时测定金银花中5种有效成分的含量。方法采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.4%的磷酸水溶液进行线性梯度洗脱,流速:1.0 mL/min,检测波长:350 nm,进样量:10μL。结果绿原酸、咖啡酸、芦丁、木犀草苷、槲皮素5个化合物在测定的范围内均表现出良好的线性关系(r≥0.999 3),回收率为97.5%～100.2%。结论该方法操作简便,分离效果好,灵敏度高,重复性好,为较全面控制金银花药材的质量提供了一种可靠的方法。

Objective To simultaneously determine the contents of five active components in Lonicera japonica Thunb by HPLC method.Methods The separation of five ingredients was achieved on a Agilent Eclipse XDB-C18 column（250 mm×4.6 mm,5 μm）with gradient elution using solvents of acetonitrile and 0.4% phosphoric acid,the flow rate was 1.0 mL/min.The detective wave length was at 350 nm,and the inpouring volume was 10 μL.Results The recovery rate was in the range of 97.5%~100.2%,and the compounds showed good linearity（r≥0.999 3）in a detection range.Conclusion The method is simple,sensitive,accurate and reproducible,it can be used to control the quality of Lonicera japonica Thunb.