乙型肝炎感染者血清标志物与ApoAI-75MspI基因多态性的相关性研究

Correlation between serum markers and polymorphism of ApoAI-75MspI gene in patients with type B hepatitis

目的探讨ApoAI-75MspI基因多态性与乙型肝炎标志物之间的内在相关性。方法留取本院720例被检者静脉血标本。为叙述方便,把HbsAg、抗-HBs、HbeAg、抗-HBe、抗-HBc分别作为1、2、3、4、5项。分为9组,1、2、3、4、5全阴组,2、5阳性组,1、3、5阳性组,1、4、5阳性组,4、5阳性组,1、5阳性组,5阳性组,2阳性组,2、4、5阳性组。每组80例。采用SnaPshot法检测ApoAI-75MspI基因的多态性,最后用测序法验证检测结果。结果 1、2、3、4、5项全阴组与其他组相比,ApoAI-75MspI等位基因和基因型分布差异有统计学意义(P<0.05)。1、2、3、4、5项全阴组G/G基因型高于其他组别,G/A基因型低于其他组别(P<0.05)。5项全阴组G等位基因频率高于其他组别(P<0.05),A等位基因频率则低于其他组别(P<0.05)。结论 ApoAI-75MspI基因多态与乙型肝炎血清标志物不同组别有相关。

Objective To explore the correlation between gene polymorphisms of ApoAI-75 MspI genotypes and models of HBV serum markers in patients with type B hepatitis.Methods Blood samples of 720 cases of subjects were collected randomly. For convenience, HbsAg, anti-HBs, HbeAg, anti-HBe, and anti-HBc were named as 1, 2, 3, 4 and 5 respectively. All patients were divided into 9 groups, including 1,2,3,4 and 5 negative group, 2 and 5 positive group, 1,3 and 5 positive group, 1,4,and 5 positive group, 4 and 5 positive group, 1 and 5 positive group, 5 positive group, 2 positive group, and 2,4 and 5 positive group. There were a total of 720 cases, including 80 patients for each group. Detection for gene polymorphisms of ApoAI-75Msp1 was carried out by single-base multilocus micro-sequencing（Snapshot method）,and different genotypes of each locus were conducted by the method of sequencing in order to support the final evidence of the accuracy of test results.Results There were significant differences not only in allele frequency but also in genotype distribution between 1,2,3,4 and 5 negative group and the rest groups. And significantly higher frequencies of G allele gene and G/G genotype were observed between 1,2,3,4 and 5 negative group and the rest groups.Conclusion The different models of HBV markers was associated with the gene polymorphism of ApoAI-75 MspI.