银翘解毒丸(大蜜丸)中牛蒡苷含量的测定

Determination of Arctiin Content in Yinqiao Jiedu Pills(Honey Pills)

目的建立以高效液相色谱法(RP-HPLC)测定银翘解毒丸中牛蒡苷含量的方法,为其质量控制提供快速、有效的分析手段。方法色谱柱为岛津C18(250 mm×4.6 mm,编号:0032140)色谱柱,柱温30℃,流动相为甲醇∶水(47∶53),流速1 mL.min-1,进样量10μL,检测波长280 nm,用外标法考察了在该色谱条件下药物的色谱峰,同时进行了专属性、线性关系、精密度等项的考察。结果牛蒡苷在0.808～2.828μg线性范围内与吸收峰面积有良好的线性关系,r=0.999 5,平均回收率为100.2%、RSD为2.82%。结论该方法专属性强,重现性好,且简便、可靠,可用于银翘解毒丸(大蜜丸)中牛蒡苷的含量测定。

Objective To establish the method of the determination of arctiin content in yinqiao jiedu pills by RP-HPLC（high performance liquid chromatography）so as to provide a rapid and effective analytic approach for the quality control.Methods Chromatographic column：Shimadzu C18（250 mm×4.6 mm,number 0032140）;column temperature：30 ℃;mobile phase：methanol∶ water（47∶ 53）;flow rate：1 mL·min-1;sample size：10 μL;wavelength：280 nm.The external standard method was adopted to investigate the chromatographic peak of the drug under the chromatographic condition.Simultaneously,the specificity,linear relationship,accuracy and the others were observed.Results Arctiin presented a good linear relationship with the absorptive peak area in the range of 0.808 to 2.828 μg,r=0.999 5,the average recovery rate=100.2% and RSD=2.82%.Conclusion This method is strong in specificity and good in repeatability.It is the simple and reliable approach for the determination of arctiin content in yinqiao jiedu pills（honey pills）.