冷冻干燥法保存人骨髓间充质干细胞的实验初探

Experimental Study on Lyophilization Preservation of Human Bone Marrow Mesenchymal Stem Cells

尝试用冷冻干燥法来保存骨髓间充质干细胞。通过差示扫描量热仪(DSC)测量样品玻璃化转变温度,从而科学确定冻干工艺温度,合理设计冷冻干燥工艺,实验实现了以30%PVP、20%海藻糖为保护液的骨髓间充质干细胞的冻干保存,其复水率达(55.2±7.66)%。该研究对解决现有的hBM-MSCs的保存瓶颈具有一定的参考价值。

The lyophilization preservation of hBM-MSCs was conducted . 30% PVP and 20% Trehalose were used as freeze-drying protectants. The vitrification temperatures of samples were measured by DSC in order to define the freeze-drying temperature scientifically. After lyophilization, hBM-MSCs were successfully resuscitated,the recovery rate reached at （55.2 ± 7.66 ）%. This work will make certain references to the researches of hBM-MSCs preservation.