氯胺酮滥用大鼠心血中氯胺酮含量变化

CHANGE OF KETAMINE CONTENT IN BLOOD OF RATS ABUSING KETAMINE

目的:研究长期腹腔注射(ip)不同剂量氯胺酮大鼠心血中氯胺酮含量变化规律。方法:120只♂Wistar大鼠随机分为4大组,分别为生理盐水对照组、10 mg.kg-1、30 mg.kg-1和60 mg.kg-1氯胺酮剂量组。每天按体重ip一次,制备大鼠氯胺酮滥用模型。对照组和3个剂量组又各分为5批,分别在染毒或给予生理盐水后45 min、1周、2周、3周和3周停药3 d后各处死一批大鼠(6只/批),提取并用GC/MS和GC法分析不同时间点大鼠心血中氯胺酮含量。结果:氯胺酮连续滥用后大鼠血中氯胺酮浓度逐渐增加,且停药3 d后仍可检出。结论:氯胺酮可能存在体内积蓄,长期服用可使氯胺酮半衰期延长。

Objective：To study the change rule of ketamine content in the blood of rats given different doses of ketamine through intraperitoneal injection（ip） for a long time.Methods：One hundred and twenty Wistar male rats were randomly divided into four large groups,physiologic saline control group,ketamine 10 mg·kg-1,30 mg·kg-1 and 60 mg·kg-1 experimental groups.The experimental groups were given ketamine hydrochloride（ip） everyday.Then the four groups were divided into five small groups（n=6）,and were executed at 45 mins,one week,two weeks,three weeks after drug administration and the third day after drug withdrawal from three week drug administration,respectively.The heart blood of rats was collected for the detection of ketamine by GC/MS and GC equipped with nitrogen phosphorus detector（NPD）.Results：The content of ketamine in rats＇ blood was increased gradually with the extension of drug administration time,and still could be detected on the third day after drug withdrawal from three week drug administration.Conclusion：Ketamine might accumulate in body and make the half-life of ketamine in body extend when ketamine is long-time abused.