EPC的变化与冠心病危险因素相关性的研究

The relationship between EPC and risk factors of coronary heart diseases

目的观察冠心病患者外周血中提取的内皮祖细胞的细胞形态、数量、成集落数量与正常对照组的区别。并研究冠心病患者EPC的数量变化与冠心病危险因素的相关性。方法选择冠心病患者57例和对照组30例,从外周血获取单个核细胞,体外培养后进行细胞分析和计数。分析冠心病患者各种危险因素与EPC细胞数量和成集落的数量的相关分析。结果①冠心病患者外周血内皮祖细胞数量和细胞集落数较对照组明显减少;②将冠心病的危险因素与EPC的数量进行相关分析发现,年龄、吸烟、高血压及LDL升高与EPC的数量明显相关。结论冠心病患者外周血内皮祖细胞的数量和成集落的数量明显降低;冠心病患者外周血内皮祖细胞的数量与冠心病危险因素相关。

[ Objective ] To investigate the isolation and culture of endothelial progenitor cells from peripheral blood in patients with coronary heart diseases, and to determine cell shape, amount and clusters. And to study the correlation of EPC number and the risk factors of coronary heart diseases. [ Methods ] Mononuclear cells were isolat- ed from peripheral blood of patients with coronary heart diseases （n =57） and control （n =30）. The isolated cells were cultured. The correlation of EPC number and the risk factors in patients suffering from coronary heart disease was investigated. [Results] The number of EPC, the number of cell clusters, migration capacity were significantly re- duced in patients with CAD, the number of EPC had significant correlation with aging, hypertension, smoking, high- LDL. [ Conclusion ] The number, the number of cell clusters, migration capacity of EPC was significantly reduced in patients with CAD compared with control subjects. The number of EPC has significant correlation with risk factors of coronary heart diseases.